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diagnoSwiss S.A.
ZI Les Ilettes, Secteur 4        -        CH-1870 MONTHEY        -        Switzerland
Phone: +41 (0)24 473 75 40        -        Fax: +41 (0)24 473 75 41         -        E-mail: info@diagnoswiss.com        -        Web: www.diagnoswiss.com

Features

  • < 15 minute ELISA
  • With antibody-coated beads:
  1. mix in tubes or micro-plate,
  2. dispense in the biosensor chip ,
  3. pipette the enzyme substrate,
  4. allow 1 minute time for readout.
  

Benefits

  • Flexibility: Bead-based protocols are easily adapted for rapid processing of most available assays.

  • Cost-efficiency: Minimal reaction chamber volume implies >10-fold reduction in ELISA kit reagent consumption which, with chip regeneration convenience, results in most competitive data- point pricing.

ELISA protocols

Three types of immunoassays have been developed, i.e.:

  • A one-step method, in which coated micro-beads, sample and detection antibody are pre- incubated (in tube), and only thereafter introduced into the micro-channel.

  • A two-step method, in which a pre-incubated mix of coated micro-beads and sample is introduced first, and detection antibody afterwards (upon pre-concentration of beads within the micro-channels).

  • A three-step method, in which coated micro-beads, sample and secondary antibody are sequentially dispensed in the biosensor chip, without prior incubation step.